Positivity of Antigen Tests Used for Diagnosis of Lymphatic Filariasis in Individuals Without Wuchereria bancrofti Infection But with High Loa loa Microfilaremia.
Identifieur interne : 000C77 ( Main/Exploration ); précédent : 000C76; suivant : 000C78Positivity of Antigen Tests Used for Diagnosis of Lymphatic Filariasis in Individuals Without Wuchereria bancrofti Infection But with High Loa loa Microfilaremia.
Auteurs : Sébastien D. Pion [France] ; Céline Montavon [France] ; Cédric B. Chesnais [France] ; Joseph Kamgno [Cameroun] ; Samuel Wanji [Cameroun] ; Amy D. Klion [États-Unis] ; Thomas B. Nutman [États-Unis] ; Michel Boussinesq [France]Source :
- The American journal of tropical medicine and hygiene [ 1476-1645 ] ; 2016.
Descripteurs français
- KwdFr :
- Adolescent, Adulte, Adulte d'âge moyen, Animaux, Antigènes d'helminthe (sang), Enfant, Enfant d'âge préscolaire, Femelle, Filariose lymphatique (diagnostic), Filariose lymphatique (parasitologie), Humains, Jeune adulte, Loa (isolement et purification), Mâle, Wuchereria bancrofti (isolement et purification).
- MESH :
- diagnostic : Filariose lymphatique.
- isolement et purification : Loa, Wuchereria bancrofti.
- parasitologie : Filariose lymphatique.
- sang : Antigènes d'helminthe.
- Adolescent, Adulte, Adulte d'âge moyen, Animaux, Enfant, Enfant d'âge préscolaire, Femelle, Humains, Jeune adulte, Mâle.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : Antigens, Helminth.
- diagnosis : Elephantiasis, Filarial.
- isolation & purification : Loa, Wuchereria bancrofti.
- parasitology : Elephantiasis, Filarial.
- Adolescent, Adult, Animals, Child, Child, Preschool, Female, Humans, Male, Middle Aged, Young Adult.
Abstract
Since the mid-2000s, the immunochromatographic card test (ICT), a point-of-care test for detecting Wuchereria bancrofti circulating filarial antigens (CFAs), has been the backbone for mapping and monitoring lymphatic filariasis (LF) worldwide. Recently, there have been instances in which CFA positivity has been associated with Loa loa microfilaremia. Here, we examined the association, at both the community and individual levels, between L. loa and CFA using additional diagnostic tools (quantitative polymerase chain reaction [qPCR], Og4C3 enzyme-linked immunosorbent assay, and IgG4 antibodies to Wb123 assays) to demonstrate the relationship between L. loa microfilaremia and ICT positivity. In May 2013, peripheral blood was collected during the day from 1,812 individuals living in southern Cameroon. ICT tests were done on the spot, and positive individuals were resampled at night. Results of qPCR and Wb123 assays concurred proving the absence of W. bancrofti infection. Og4C3 assays indicate a quantitative relationship between the level of L. loa microfilaremia and that of CFA. This was confirmed by epidemiological analyses, which reveal a strong association between L. loa microfilaremia and ICT positivity, with 50% of ICT reacting to L. loa when its microfilarial density exceeds 30,000 microfilariae/mL. At the community level, the proportion of positive ICT would exceed 2% when the prevalence of L. loa microfilaremia in the total population is above 20%. This has significant implications in terms of mapping and control of LF caused by W. bancrofti in Loa-endemic areas. Cross-reactivity of ICT with L. loa has to be considered in the context of both individual and community diagnostics.
DOI: 10.4269/ajtmh.16-0547
PubMed: 27729568
Affiliations:
- Cameroun, France, États-Unis
- Languedoc-Roussillon, Maryland, Occitanie (région administrative)
- Montpellier
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Le document en format XML
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Pion</name><affiliation wicri:level="3"><nlm:affiliation>Unité Mixte Internationale 233, Institut de Recherche pour le Développement, Université de Montpellier, Institut National de la Santé et de la Recherche Médicale, Montpellier, France. sebastien.pion@ird.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Unité Mixte Internationale 233, Institut de Recherche pour le Développement, Université de Montpellier, Institut National de la Santé et de la Recherche Médicale, Montpellier</wicri:regionArea><placeName><region type="region">Occitanie (région administrative)</region><region type="old region">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author><author><name sortKey="Montavon, Celine" sort="Montavon, Celine" uniqKey="Montavon C" first="Céline" last="Montavon">Céline Montavon</name><affiliation wicri:level="3"><nlm:affiliation>Unité Mixte Internationale 233, Institut de Recherche pour le Développement, Université de Montpellier, Institut National de la Santé et de la Recherche Médicale, Montpellier, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Unité Mixte Internationale 233, Institut de Recherche pour le Développement, Université de Montpellier, Institut National de la Santé et de la Recherche Médicale, Montpellier</wicri:regionArea><placeName><region type="region">Occitanie (région administrative)</region><region type="old region">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author><author><name sortKey="Chesnais, Cedric B" sort="Chesnais, Cedric B" uniqKey="Chesnais C" first="Cédric B" last="Chesnais">Cédric B. 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Nutman</name><affiliation wicri:level="2"><nlm:affiliation>National Institutes of Health, Bethesda, Maryland.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>National Institutes of Health, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Boussinesq, Michel" sort="Boussinesq, Michel" uniqKey="Boussinesq M" first="Michel" last="Boussinesq">Michel Boussinesq</name><affiliation wicri:level="3"><nlm:affiliation>Unité Mixte Internationale 233, Institut de Recherche pour le Développement, Université de Montpellier, Institut National de la Santé et de la Recherche Médicale, Montpellier, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Unité Mixte Internationale 233, Institut de Recherche pour le Développement, Université de Montpellier, Institut National de la Santé et de la Recherche Médicale, Montpellier</wicri:regionArea><placeName><region type="region">Occitanie (région administrative)</region><region type="old region">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author></analytic><series><title level="j">The American journal of tropical medicine and hygiene</title><idno type="eISSN">1476-1645</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Animals</term><term>Antigens, Helminth (blood)</term><term>Child</term><term>Child, Preschool</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Elephantiasis, Filarial (parasitology)</term><term>Female</term><term>Humans</term><term>Loa (isolation & purification)</term><term>Male</term><term>Middle Aged</term><term>Wuchereria bancrofti (isolation & purification)</term><term>Young Adult</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adolescent</term><term>Adulte</term><term>Adulte d'âge moyen</term><term>Animaux</term><term>Antigènes d'helminthe (sang)</term><term>Enfant</term><term>Enfant d'âge préscolaire</term><term>Femelle</term><term>Filariose lymphatique (diagnostic)</term><term>Filariose lymphatique (parasitologie)</term><term>Humains</term><term>Jeune adulte</term><term>Loa (isolement et purification)</term><term>Mâle</term><term>Wuchereria bancrofti (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antigens, Helminth</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Loa</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Loa</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Antigènes d'helminthe</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Animals</term><term>Child</term><term>Child, Preschool</term><term>Female</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Young Adult</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adolescent</term><term>Adulte</term><term>Adulte d'âge moyen</term><term>Animaux</term><term>Enfant</term><term>Enfant d'âge préscolaire</term><term>Femelle</term><term>Humains</term><term>Jeune adulte</term><term>Mâle</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Since the mid-2000s, the immunochromatographic card test (ICT), a point-of-care test for detecting Wuchereria bancrofti circulating filarial antigens (CFAs), has been the backbone for mapping and monitoring lymphatic filariasis (LF) worldwide. Recently, there have been instances in which CFA positivity has been associated with Loa loa microfilaremia. Here, we examined the association, at both the community and individual levels, between L. loa and CFA using additional diagnostic tools (quantitative polymerase chain reaction [qPCR], Og4C3 enzyme-linked immunosorbent assay, and IgG4 antibodies to Wb123 assays) to demonstrate the relationship between L. loa microfilaremia and ICT positivity. In May 2013, peripheral blood was collected during the day from 1,812 individuals living in southern Cameroon. ICT tests were done on the spot, and positive individuals were resampled at night. Results of qPCR and Wb123 assays concurred proving the absence of W. bancrofti infection. Og4C3 assays indicate a quantitative relationship between the level of L. loa microfilaremia and that of CFA. This was confirmed by epidemiological analyses, which reveal a strong association between L. loa microfilaremia and ICT positivity, with 50% of ICT reacting to L. loa when its microfilarial density exceeds 30,000 microfilariae/mL. At the community level, the proportion of positive ICT would exceed 2% when the prevalence of L. loa microfilaremia in the total population is above 20%. This has significant implications in terms of mapping and control of LF caused by W. bancrofti in Loa-endemic areas. Cross-reactivity of ICT with L. loa has to be considered in the context of both individual and community diagnostics.</div></front></TEI><affiliations><list><country><li>Cameroun</li><li>France</li><li>États-Unis</li></country><region><li>Languedoc-Roussillon</li><li>Maryland</li><li>Occitanie (région administrative)</li></region><settlement><li>Montpellier</li></settlement></list><tree><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Pion, Sebastien D" sort="Pion, Sebastien D" uniqKey="Pion S" first="Sébastien D" last="Pion">Sébastien D. Pion</name></region><name sortKey="Boussinesq, Michel" sort="Boussinesq, Michel" uniqKey="Boussinesq M" first="Michel" last="Boussinesq">Michel Boussinesq</name><name sortKey="Chesnais, Cedric B" sort="Chesnais, Cedric B" uniqKey="Chesnais C" first="Cédric B" last="Chesnais">Cédric B. Chesnais</name><name sortKey="Montavon, Celine" sort="Montavon, Celine" uniqKey="Montavon C" first="Céline" last="Montavon">Céline Montavon</name></country><country name="Cameroun"><noRegion><name sortKey="Kamgno, Joseph" sort="Kamgno, Joseph" uniqKey="Kamgno J" first="Joseph" last="Kamgno">Joseph Kamgno</name></noRegion><name sortKey="Wanji, Samuel" sort="Wanji, Samuel" uniqKey="Wanji S" first="Samuel" last="Wanji">Samuel Wanji</name></country><country name="États-Unis"><region name="Maryland"><name sortKey="Klion, Amy D" sort="Klion, Amy D" uniqKey="Klion A" first="Amy D" last="Klion">Amy D. Klion</name></region><name sortKey="Nutman, Thomas B" sort="Nutman, Thomas B" uniqKey="Nutman T" first="Thomas B" last="Nutman">Thomas B. Nutman</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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